Proteomics Primer 1. Proteomics 2. 2-D PAGE 3. Immobilised pH gradients (IPGs) 4. Mass spectrometry 5. Principles of mass spectrometry 6. Matrix assisted laser desorption/ionisation (MALDI) 7. Electrospray ionisation 8. Mass Analysers?- Time-of-flight analysers 9. Mass Analysers - Quadrupole mass analyser??? 10. Application of MALDI-TOF in proteomics 11. Protein identification???? 12. Study of proteins and peptides by ESI 13. Tandem mass spectrometry 14. Post source decay (PSD) 15. Sequencing of peptides using PSD 16. Tandem mass spectrometry using electrospray ionisation 17. Protein identification using ESI-MS/MS 18. Hybrid quadrupole-TOF (Q-TOF) for MS/MS 19. Protein-Protein interactions 20. Mass spectrometric identification of complexes 21. Alternatives to 2-D PAGE 22. References