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化学生物学实验技术>Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine A
Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine A
关键词: amino acid analysis来源: 互联网
Precolumn derivatization of amino acids followed by their resolution by reverse-phase high-performance liquid chromatography (RP-HPLC) is now the preferred method for quantitative amino acid analysis. The derivatization step introduces covalently bound chromophores necessary not only for interactions with the apolar stationary phase for high resolution, but also for photometric or fluorometric detection.
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