Assessment of TNFα-Induced Endothelial Damage Through the Loss of Its Barrier Function
Tumor necrosis factor α (TNFα) appears to be the main mediator causing the systemic manifestations of sepsis and septic shock associated with edema (1 ,2 ). In vitro it augments movement of permeability tracers across endothelial cell monolayers (3 ,4 ). Herein we describe a method which permits quantification and assessment of changes in the integrity of endothelial monolayers by measuring transendothelial flux (luminal to subluminal) of tracer macromolecules in the absence of hydrostatic and oncotic pressure gradients. The system consists of two compartments separated by a polycarbonate filter and permits highly controlled conditions.
- Instrumentation, Accuracy, and Quality Control Issues in Development of Quantitative Fluorescence-Image Analysis (QFIA)
- Image Analysis Using the Fluorochromasia Assay to Quantify Tumor Drug Sensitivity
- Colloidal Gold: A Novel Nanoparticle for Targeted Cancer Therapeutics
- Antisense IGF-I for Hepatocellular Carcinoma
- Green Fluorescent Protein for Metastasis Research
- Targeting the Mitochondria to Enhance Tumor Suppression
- 2-DE-Based Proteomics for the Analysis of Metastasis-Associated Proteins
- Differential Display Techniques to Identify Tumor Suppressor Gene Pathways
- cos-7的转染
- Molecular Characterization of Inflammation-Induced JNK/c-Jun Signaling Pathway in Connection with Tumorigenesis