Introduction to Cell Culture
The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture. Steps must be taken to maintain aseptic conditions to prevent contamination of cultures with micro-organisms. Basic cell-handling techniques are discussed, including choice of media, primary culture, and cryopreservation of cells so they can be stored for future use. Common assays which are used to determine cell viability and activity are considered.
- 次缢痕(secondary constriction)
- In Vivo Transplantation and Tooth Repair
- Methyl-Combing: Single-Molecule Analysis of DNA Methylation on Stretched DNA Fibers
- Serial Cultivation of Primary Adult Murine Keratinocytes
- Fluorescent Differential Display
- ATP-Loaded Liposomes for Targeted Treatment in Models of Myocardial Ischemia
- 细胞通讯(cell communication)
- An In Vitro Assay for the Kinase Activity of mTOR Complex 2
- Detecting sRNAs by Northern Blotting
- Claudin-4: Functional Studies Beyond the Tight Junction