Switching on Lineage Tracers Using Site-Specific Recombination
Methods to study the establishment and distribution of embryonic cell lineages have increased our understanding of the diverse events that comprise normal development. The resultant fate maps have also provided an important framework for systematically analyzing genotype-phenotype relationships uncovered by mutagenesis. Until recently, vertebrate fate maps have been plotted principally in avian systems because of the ease of manipulating tissue in ovo. These studies, using methods comprised of injecting retroviral (1 ,2 ) or fluorescent lineage tracers (3 ) or of grafting quail cells into chick embryos (4 ), have provided the core of what we know about vertebrate development. In contrast to chicken embryos developing in eggs, mouse embryos developing in utero are much less accessible, making the established tracing methods significantly more difficult. To circumvent these difficulties, noninvasive methods have recently been developed to genetically activate lineage tracers in mice using site-specific recombination (5 ,6 ).
- Primary Kidney Proximal Tubule Cells
- Protection of Purified Human Hematopoietic Progenitor Cells by Interleukin-1 or Tumor Necrosis Factor-
- Flow Cytometry of Fibroblast Nuclei for DNA content
- Silencing of Rho-GDI by RNAi Promotes the Differentiation of Neural Stem Cells
- Methods for Cancer Stem Cell Detection and Isolation
- Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media
- Purification and Culture of Fetal Mouse Hepatoblasts that Are Precursors of Mature Hepatocytes and Biliary Epithelial Cells
- Site-Specific Cleavage of Fusion Proteins
- Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins
- Simultaneous analysis of DNA content and surface