Isolation and Maintenance of Mouse Epiblast Stem Cells
关键词: epiblast stem cells来源: 互联网
Epiblast stem cells (EpiSCs) are isolated from the postimplantation mouse embryo just after implantation but prior to gastrulation. EpiSCs are pluripotent and provide a tractable, in vitro system to study the processes that function during gastrulation to transition pluripotent cells to their differentiated derivatives. This chapter describes the methods for the isolation and maintenance of mouse EpiSCs. We also describe basic assays used to characterize new EpiSC lines.
推荐方法
- Direct DNA Sequencing of PCR Products Using Magnetic Beads
- 组织工程(histological engineering)
- Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
- The Purification of Mouse Hematopoietic Stem Cells at Sequential Stages of Maturation
- Mouse Chimeras and the Analysis of Development
- Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes
- Proteomics Studies After Hematopoietic Stem Cell Transplantation
- 犬白细胞介素 1(IL-1)酶联免疫分析(ELISA)
- 细胞的冷冻保存
- 线粒体的活体染色及电镜照片观察