Comparative Genomic Hybridization for the Analysis of Unbalanced Chromosomal Abnormalities in Ovarian Tumors
Comparative genomic hybridization (CGH) is a powerful technique for the quantitative detection of changes in chromosome copy number (1 -3 ). It offers an advantage over conventional cytogenetic techniques in the analysis of tumor karyotypes through its utilization of DNA as the material of study, thus avoiding the problems associated with obtaining good quality metaphases from tumor material. Though CGH is not sensitive to balanced chromosomal rearrangements and changes in DNA ploidy, it can be sensitive to twofold differences in chromosome copy number, making identification of mono- and trisomic karyotype straightforward. Furthermore, deletions and amplifications as small as 2 Mb can be detected, and identification of such regions by CGH analyses of ovarian cancers (4 -6 ) have already contributed to the identification of the putative oncogene PIK3CA (7 ).
- Gene Expression Profiling in Cervical Cancer: Identification of Novel Markers for Disease Diagnosis and Therapy
- Epidemiology of Human Papilloma Virus (HPV) in Cervical Mucosa
- Sensitive Detection of Hypermethylated p16INK4a Alleles in Exfoliative Tissue Material
- SPR1: An Early Molecular Marker for Bronchial Carcinogenesis
- Clinical, Pathologic, and Imaging Features and Biological Markers of Uveal Melanoma
- PNAS:真核生物囊泡转运机理新思路
- 正常人胚肺成纤维细胞的培养
- 甲醇酵母表达注意事项
- 数据的显示与分析
- Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells