In Vivo Germ Line Stem Cell Migration: A Mouse Model
A stem cell niche is a specialized tissue environment that controls the proliferation and differentiation of its resident stem cells. The functions of these structures have been well characterized in adult organisms. In particular, the bone marrow stem cell niche in mammals has been amenable to analysis because of the ability of transplanted hematopoietic cells to home and to recolonize the bone marrow of an irradiated host. Despite clues from adult models, it remains unclear how stem cells become partitioned into appropriate niches during embryonic development. To examine the earliest steps in niche formation, we created an organ culture system to observe the development of primordial germ cells (PGCs), a migratory stem cell population that will eventually give rise to the gametes. Using this assay, we can watch PGCs as they migrate to colonize the developing gonads and can introduce growth factor agonists or antagonists to test the function of proteins that regulate this process. This provides an unprecedented opportunity to identify the cellular and molecular interactions required for the formation of the germ cell niche.
- Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy
- Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
- 关于细胞培养中支原体污染的经验分享
- The Application of Differential Display to the Brain: Adaptations for the Study of Heterogeneous Tissue
- Studying Vascular Progenitor Cells in a Neonatal Mouse Model
- Fibrin Gel Model for Assessment of Cellular Contractility
- Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice
- 高尔基复合体(Golgi complex)
- 结晶紫法检测细胞增殖
- 内吞作用