Cryopreservation of Semen from Domestic Livestock
Although the first observations concerning low temperature preservation of spermatozoa date back to 1776 (when Spallanzani noted that spermatozoa, cooled in snow, became inactive but were revived on warming), successful cryopreservation protocols truly date from only the 1940s and 1950s. Around this time semen preservation was in the forefront of the new science of cryobiology and was important in the formulation of the first theories concerning the causes of cryoinjury. In 1938 Jahnel noted the survival of human spermatozoa stored at the temperature of solid carbon dioxide, an observation taken up by Parkes in 1945 (1 ), but the single most important development came with the discovery by Polge, Smith, and Parkes in 1949 (2 ) that glycerol could act as a cryoprotectant for spermatozoa. The initial experiments with glycerol were performed using fowl spermatozoa but were quickly followed by the successful preservation of bull spermatozoa (3 ) with the first calf from artificial insemination (AI) using frozen/thawed spermatozoa reported in 195l (4 ). However, early success with the bull rather belied the difficulties that became apparent with other domestic species. The use of glycerol had allowed bull spermatozoa to survive at the comparatively slow freezing rates obtained using methanol cooled with solid CO2 , and with semen contained in large glass-freezing ampules.
- Current Status of the Polyamine Research Field
- Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis
- 小鼠白细胞介素-35(IL-35)酶联免疫分析(ELISA)
- Activation of the Mitogen-Activated Protein Kinase Pathway by TGF
- Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes
- Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Postm
- Purifying the Hedgehog Protein and its Variants
- ProteinProtein Interaction Techniques: Dissect PCP Signaling in Xenopus
- PTEN介导的细胞生长抑制和细胞凋亡信号图
- Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies