Assays for the Evaluation of HIV-1 Integrase Inhibitors
Integration is an essential step in the replication cycle of a retrovirus, such as the human immunodeficiency virus type 1 (HIV-1) (for a review, see ref . 1 ). After reverse transcription of the RNA genome, the DNA copy is transported into the nucleus and integrated in the host chromosome. The only viral enzyme required for HIV-1 integration is integrase (IN), a protein of 32 kDa encoded by the 3′-end of the pol gene. The enzyme is produced by protease-mediated cleavage of the gag-pol precursor during virion maturation. Integrase can be considered a site-specific endonuclease; the removal of two nucleotides creates a hydroxyl residue at each 3′-end of the viral DNA that will carry out a nucleophilic attack on the phosphodiester backbone of the host DNA.
- Development of Physiological Models to Study Stress Protein Responses
- Designing, Testing, and Validating a Microarray for Stem Cell Characterization
- Electron Microscopy and In Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum
- 核小体(nucleosome)
- Generating Neurons from Stem Cells
- Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations
- Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration
- Chromatin Immunoprecipitation Assays for Myc and N-Myc
- Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins
- 酵母遗传学方法9:酵母免疫荧光