Cell Culture Contamination
Microbial contamination is a major issue in cell culture, but there are a range of procedures which can be adopted to prevent or eliminate contamination. Contamination may arise from the operator and the laboratory environment, from other cells used in the laboratory, and from reagents. Some infections may present a risk to laboratory workers: containment and aseptic technique are the key defence against such risks. Remedial management of suspected infection may simply mean discarding a single potentially infected culture. However, if a more widespread problem is identified, then all contaminated cultures and associated unused media that have been opened during this period should be discarded, equipment should be inspected and cleaned, cell culture operations reviewed, and isolation from other laboratories instituted until the problem is solved. Attention to training of staff, laboratory layout, appropriate use of quarantine for new cultures or cell lines, cleaning and maintenance, and quality control are important factors in preventing contamination in cell culture laboratories.
- Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics
- Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis
- Cytogenetic Methods in Chronic Lymphocytic Leukemia
- Fluorescence In Situ Hybridization and Comparative Genomic Hybridization
- Methylation Analysis of CpG Islands
- Epidemiological Investigation of Prostate Cancer
- Glycoproteomic Analysis by Two-Dimensional Electrophoresis
- Hybrid Capture of Putative Tumor Suppressor Genes
- Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR
- Cationic Liposome Gene Transfer