Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line
Cell culture is an invaluable tool for investigators in numerous fields. It facilitates analysis of biological properties and processes that are not readily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, requires knowledge and practice of a few essential techniques. The purpose of this chapter is to explain the basic principles of cell culture using the maintenance of a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples. Procedures for thawing, culture, determination of cell numbers and viability, and cryopreservation are described.
- Future and Applications of Cloning
- Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections
- The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures
- Identification of Osteoclasts in Culture
- Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombe
- Human Mesenchymal Stem Cell Culture for Neural Transplantation
- Live Imaging Mouse Embryonic Development: Seeing Is Believing and Revealing
- Capillary Electrophoresis for Pharmaceutical Analysis
- 细胞株培养细胞
- 表皮生长因子(epidermal growth factor, EGF)