热门搜索词：
Isolation of RNA From Tumor Samples: Single-Step Guanidinium Acid-Phenol Method
关键词： isolation from tumor来源： 互联网
The guanidinium acid-phenol method of RNA extraction is relatively fast (4 h) and is useful for the processing of large numbers of samples, without the need for ultracentrifugation. This protocol produces total RNA that includes ribosomal, transfer, and messenger RNA.
This high-quality RNA is suitable for Northern blot analysis, dot-blot hybridization, poly (A) RNA selection, in vitro translation, cDNA library construction, reverse transcriptase-polymerase chain reaction, ribonuclease protection assay, and primer extension experiments.
推荐方法
- Integrative Prediction of Gene Function and Platinum-Free Survival from Genomic and Epigenetic Features in Ovarian Cancer
- Methods for Analyzing Checkpoint Responses in Caenorhabditis elegans
- The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress
- Conventional Cytogenetics in Myeloma
- Analysis of Cell Cycle Progression and Genomic Integrity in Early Lethal Knockouts
- Primary Surgical Management of Ovarian Cancer
- Isolation and Culture of Renal Cancer Cell Lines
- Utility of Fine-Needle Aspiration Biopsy for Prospective Analysis of Patients Undergoing Therapy for Metastatic Melanoma
- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR
- On-Demand Cleavable Linkers for Radioimmunotherapy
移动版：