Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a Syste
Lung primordial specification as well as branching morphogenesis, and the formation of various pulmonary cell lineages, requires a specific interaction of the lung endoderm with its surrounding mesenchyme and mesothelium. Lung mesenchyme has been shown to be the source of inductive signals for lung branching morphogenesis. Epithelial–mesenchymal–mesothelial interactions are also critical to embryonic lung morphogenesis. Early embryonic lung organ culture is a very useful system to study epithelial–mesenchymal interactions. Both epithelial and mesenchymal morphogenesis proceed under specific conditions that can be readily manipulated in this system (in the absence of maternal influence and blood flow). More importantly this technique can be readily done in a serumless, chemically defined culture media. Gain and loss of function can be achieved using expressed proteins, recombinant viral vectors, and/or analysis of transgenic mouse strains, antisense RNA, as well as RNA interference gene knockdown. Additionally, to further study epithelial–mesenchymal interactions, the relative roles of epithelium versus mesenchyme signaling can also be determined using tissue recombination (e.g., epithelial and mesenchymal separation) and microbead studies.
- Three-Dimensional Interphase Analysis Enabled by Suspension FISH
- From ES Cells to Mice: The Gene Trap Approach
- Steroid Hormone Metabolites and Hormone Binding Assays
- 接触抑制(contact inhibition)
- Coculture of Decidua and Trophoblast to Study Proliferation and Invasion
- Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent
- Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins
- 结构性异染色质(constitutive heterochromatin)
- 293细胞特性概述
- P位点(P site)