The process of carcinogenesis involves a number of changes in cellular phenotype, which are largely based on acquired genetic changes in cells that are not terminally differentiated. The ability of cancer cells to grow and their failure to respond to the usual controls on such proliferation are obvious features, but they also evade cell death and most have no limits on their ability to replicate beyond the limits imposed by telomere length in normal cells. In addition, they are able to stimulate the formation of blood vessels to ensure a steady supply of oxygen and nutrients, and to invade normal tissues, sometimes subverting the normal processes within those tissues. Finally, it has become increasingly apparent that cancer cells undergo a process of selection which renders the immune system ineffective. Some of these characteristics are retained by cells in culture, and an understanding of the biological properties of cancer cells will assist in the design of experiments and the interpretation of their results.