Flow Cytometric Detection of Activated Caspase-3
Apoptosis (programmed cell death) is an active process that plays a critical role in multiple biologic processes from embryologic development, to lymphocyte development and selection, and homeostasis. The two major mechanisms of cell death are referred to as the intrinsic and extrinsic pathways. These pathways lead to a cascade of events that ultimately converge to the activation of an effector enzyme, caspase-3. Caspase-3 is a cysteine protease with aspartic specificity and a well-characterized effector of apoptosis or programmed cell death signaling. The pro-form of caspase-3 (p32 caspase-3) is sequestered as a zymogen, where upon proteolysis at a conserved DEVD sequence, is converted to the active (p17 caspase-3) enzyme capable of disassembling the cell. Cell death can become disregulated under various conditions and multiple disease states (e.g., viral infection, carcinogenesis, and metastasis). Sensitive and reproducible detection of active caspase-3 is critical to advance the understanding of cellular functions and multiple pathologies of various etiologies. Here, we provide two simple and reproducible methods to measure active caspase-3 in multiple cell types and conditions using a flow cytometric-based analysis.
- Use of Nanoparticles for Targeted, Noninvasive Thermal Destruction of Malignant Cells
- The Prevention and Genetics of Pancreatic Cancer: A Programmatic Approach
- FISH Techniques
- Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
- Determination of Cell-Specific Receptor Binding Using a Combination of Immunohistochemistry and In Vitro Autoradiography: Releva
- Measuring MDR-1 by Quantitative RT-PCR
- 转染细胞不表达怎么办
- MHC 四聚体助力细胞治疗基础研究:CTL 表位筛选
- Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas
- 外源基因在真核细胞表达步骤