Isolation and Culture of Pig Epiblast Stem Cells
关键词: isolation culture pig来源: 互联网
Regulation of early development can be directly interrogated in the embryo or can be studied in cultured cells isolated in the laboratory. New understanding of the developmental stages and the signalling requirements of the pig embryo have enabled the development of improved protocols for the derivation of pluripotent cells from early epiblasts. Here, we provide a detailed step-by-step description of the critical parameters required for isolation and establishment of these cells and how they can be used to study their developmental properties.
推荐方法
- Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli
- Radio-Isotopic In Situ Hybridization on Tissue Sections: Practical Aspects and Quantification
- The Generation of Embryoid Bodies from Feeder-Based or Feeder-Free Human Pluripotent Stem Cell Cultures
- Peptide Structure Determination by NMR
- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR
- Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins
- The 25A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI)
- Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing
- Preparing and using bacterial cells for electroporation
- Autoradiography of 1-and 2-Adrenoceptors