Mesenchymal Stem Cell Therapy on Murine Model of Nonalcoholic Steatohepatitis
A severely malfunctioning liver, due to acute liver injury or chronic liver disease, can lead to hepatic failure. The ultimate treatment for hepatic failure is liver transplantation; however, the availability of donors is a critical issue. Therefore, regenerative therapy is an anticipated novel approach for restoring liver function. Mesenchymal stem cells are pluripotent somatic cells that can differentiate into several cell types, including hepatocytes. Moreover, they are obtainable from easily accessible autologous adipose tissue, making them ideal for regenerative therapy. This chapter describes experimental methods for isolating mesenchymal stem cells from murine adipose tissues and expanding them, and also describes murine chronic liver disease, steatohepatitis, for the study of experimental regenerative treatments of chronic liver disease.
- Techniques for Thick-Section Golgi Impregnation of Formalin-Fixed Brain Tissue
- Three-Dimensional Interphase Analysis Enabled by Suspension FISH
- ALDH as a Marker for Enriching Tumorigenic Human Colonic Stem Cells
- Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
- Adhesion to Matrix Proteins
- Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements
- Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes
- 动物体内转染试剂
- 膜骨架(membrane skeleton)
- 大鼠内皮细胞的培养方案