Determining Mutational Status of Immunoglobulin V Genes in Chronic Lymphocytic Leukemia: A Useful Prognostic Indicator
Determining the clonal origins of malignant B-cells will have an impact on disease understanding and management. In this regard, immunoglobulin variable (V) region gene analysis already is having a significant impact in delineating the tumor cell of origin. It can identify, among other features whether such a cell has undergone somatic mutation, which usually occurs within germinal centres. Remarkably, in chronic lymphocytic leukemia (CLL), the mutational status of V genes has allowed researchers to identify two subsets of disease, one originating from an unmutated B-cell with a markedly poorer disease outcome and the other from a mutated B-cell, which associates with long-term survival. The V gene status in CLL thus provides a robust indicator of disease outcome, which is beginning to shape clinical treatment. This chapter describes in detail the methodology for determining V gene usage in CLL, from acquisition of patient sample to generating the V-gene readout.
- Construction and Characterization of an Oncolytic HSV Vector Containing a Fusogenic Glycoprotein and Prodrug Activation for Enha
- The Use of Early Sea Urchin Embryos in Anticancer Drug Testing
- Establishment of Primary Cultures from Ovarian Tumor Tissue and Ascites Fluid
- Investigating Macrophage and Malignant Cell Interactions In Vitro
- Cytokine Multiplex Analysis
- Markers of Tumor Burden: An Overview
- 7 In Vitro Methods for Evaluation of P450-Based Anticancer Gene Therapy
- Multiple Myeloma: Challenges and Opportunities
- Detection of Telomerase hTERT Gene Expression and Its Splice Variants by RT-PCR
- galactosidase assay for cells transfected with pSV- -Galactosidase Vector