Studying Mycs Role in Metabolism Regulation
The MYC oncogene encodes a master transcription factor, Myc, which regulates genes involved in ribosome biogenesis, lipid synthesis, nucleic acid synthesis, intermediary metabolism, and cell growth and proliferation. The genomics of Myc target genes has been well-established through global mapping of Myc binding sites in a variety of different cancer cell lines. These studies highlight the importance of Myc in regulating glucose and glutamine metabolism as well as mitochondrial and ribosomal biogenesis. These genomic studies, however, only become relevant with the companion metabolic studies using a variety of methods to measure oxygen consumption, glucose uptake, or metabolic pathways based on 13 C-labeled glucose or glutamine uptake. These methods are described herein.
- Isolation and Characterization of Side Population Cells
- Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA
- Isolation and Characterization of Human Fetal Myoblasts
- Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases
- Mapping the Lipolytic Proteome of Adipose Tissue Using Fluorescent Suicide Inhibitors
- Isolation and Characterization of the Heat Shock RNA 1
- Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisa
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