CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements
Most cyclin-dependent kinases are negatively regulated by phosphorylation of two residues, a threonine at residue 14 and a tyrosine at residue 15. These residues are dephosphorylated by the cdc25 family of dual-specificity phosphatases leading to cell cycle progression. These phosphatases are inactivated by cellular checkpoint pathways in response to DNA damage leading to cell cycle arrest. Checkpoint pathways regulate the function of these phosphatases by regulating their stability, localization, association with substrate, and their activity. Hence, determining these properties for the cdc25 family of phosphatases becomes crucial for understanding how checkpoint pathways regulate the function of the cdc25 family members and, hence, cell cycle progression. This chapter describes methods to determine the activity, levels, phosphorylation status, and localization of both endogenous and overexpressed cdc25 proteins.
- 水与平衡盐溶液
- Analyzing p53 Regulated DNA Damage Checkpoints by Flow Cytometry
- Detection of Activated STAT Proteins
- Establishment and Maintenance of Normal Human Keratinocyte Cultures
- Bistability in One Equation or Fewer
- Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells
- The Dansyl-Edman Method for Peptide Sequencing
- Monitoring Blood for CD34+ Cells to Determine Timing of Hematopoietic Progenitor Cells Apheresis
- Cr Release Cytotoxicity assay
- 利用细胞计数板测定血细胞数量及大小