Studying Mechanosensitive Ion Channels Using Liposomes
Mechanosensitive (MS) ion channels are the primary molecular transducers of mechanical force into electrical and/or chemical intracellular signals in living cells. They have been implicated in innumerable mechanosensory physiological processes including touch and pain sensation, hearing, blood pressure control, micturition, cell volume regulation, tissue growth, or cellular turgor control. Much of what we know about the basic physical principles underlying the conversion of mechanical force acting upon membranes of living cells into conformational changes of MS channels comes from studies of MS channels reconstituted into artificial liposomes. Using bacterial MS channels as a model, we have shown by reconstituting these channels into liposomes that there is a close relationship between the physico-chemical properties of the lipid bilayer and structural dynamics bringing about the function of these channels.
- Methyl-Combing: Single-Molecule Analysis of DNA Methylation on Stretched DNA Fibers
- 中期(metaphase)
- PACE Analysis of RNA-Peptide Interactions
- Modeling Brain Pathologies Using Neural Stem Cells
- Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS
- Intravenous Human Mesenchymal Stem Cells Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage
- Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias latipes
- Markers of Cellular Senescence
- Identifying Different Types of Chromatin Using Giemsa Staining
- 激光显微细胞分离技术