Detection of Apoptosis in Drosophila
Drosophila has unique genetic and cell biological advantages as a model system for the study of apoptosis. Many cell death genes are evolutionarily conserved between flies and mammals. Cell death can be induced by environmental stimuli and normally occurs during diverse developmental processes in Drosophila . Here, we review several approaches for detecting cell death in Drosophila . We provide detailed protocols for labeling apoptotic cells in the embryo and ovary using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and acridine orange. Additionally, we describe methods for ectopically expressing cell death genes in the eye and the use of transgenic flies for the detection of genetic interactions among cell death genes.
- Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant Unilamellar Vesicles
- MT Spindowns from Extracts
- 成虫盘(imaginal discs)
- Cryopreservation of Mouse Embryos
- 人胃动素(MTL)酶联免疫分析(ELISA)
- In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
- PTEN介导的细胞生长抑制和细胞凋亡信号图
- DNA疫苗实验方法
- 受体介导的内吞作用(receptor-mediated endocytosis)
- 减数分裂过程的识别