-Tubulin and Microtubule-Binding Assays
Dynamic instability is a hallmark of the microtubule cytoskeleton. In order to regulate microtubule dynamics in vivo, a varied host of microtubule-associated proteins are mobilized to promote microtubule nucleation, growth, stabilization, catastrophe, depolymerization, rescue, and severing. To confer these various functions, cytoskeletal regulators have highly tuned affinities for tubulin, recognizing the unpolymerized αβ-tubulin heterodimer, dynamic microtubule lattice, stabilized microtubule lattice, or a combination therein. The protocols presented here probe αβ-tubulin and microtubule binding using gel filtration and cosedimentation, respectively.
- Preparation of cytoplasmic extracts for the application in a cell-free system
- Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells
- Unbiased Characterization of the Senescence-Associated Secretome Using SILAC-Based Quantitative Proteomics
- 联会复合体的染色与观察
- Isolation of Messenger RNA
- Live Cell Fluorescence Microscopy Techniques
- Detection and Quantification of Cytokines and Other Biomarkers
- 减数分裂实验
- Strategies to Generate Induced Pluripotent Stem Cells
- 细胞裂解液的配置方法