Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)
We have developed protocols whereby a total of 30–90 � 106 hMSCs with an average viability greater than 90% can be produced in a single multilevel Cell Factory from a relatively small (1–3 mL) bone marrow aspirate in 14–20 d. It is possible to generate as many as 5 � 108 multipotent stromal cells (MSCs) from a single sample, depending on the number of Cell Factories seeded from the initial isolated hMSCs. Briefly, mononuclear cells are collected from a bone marrow aspirate by density gradient centrifugation. The cells are cultured overnight and the adherent cells are allowed to attach to the flask. Nonadherent cells are removed and the culture expanded for 7–10 d with periodic feeding of the cells. The cells are then harvested and seeded at low density (60–100 cells/cm2 ) into Nunc Cell Factories. The cells are allowed to expand for an additional 7–10 d, and are then harvested.
- Solid Phase Assays for Studying ECM ProteinProtein Interactions
- Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract
- Single Nucleotide Polymorphism Genotyping in MMP Genes: The 5 Nuclease Assay
- Transdermal Delivery Using Surface Electrodes in Porcine Skin
- New Protocol for Lentiviral Vector Mass Production
- Induction of Deoxyribonucleic Acid Damage by Irradiation
- Synchronization of Mammalian Cell Cultures by Serum Deprivation
- Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells
- 血吸虫型肝硬化模型制备
- Quantitation of Argatroban in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (UPLC-ESI-MS/MS)