Chemokine Receptor Dimerization and Chemotaxis
A broad array of biological responses ranging from cell polarization, movement, immune and inflammatory responses, as well as prevention of HIV-1 infection, are triggered by the chemokines, a family of structurally related chemoattractant proteins that bind to specific seven-transmembrane receptors linked to G proteins. Although it was initially believed that chemokine receptors act as monomeric entities, it has now been shown that they function as oligomers. Chemokine receptor homo– and heterodimers are found on the cell membrane; binding to their ligands stabilizes specific receptor conformations and activates distinct signaling cascades. Thorough analysis of the conformations adopted by the receptors at the membrane is therefore a prerequisite for understanding the function of these inflammatory mediators. For study of the chemokine receptor conformations at the cell surface, we focus here on conventional biochemical and genetic methods, as well as on new imaging techniques such as those based on resonance energy transfer; we also evaluate in vitro and in vivo methods to determine certain chemokine receptor functions.
- 细胞培养板的选择
- Inducing High Rates of Targeted Mutagenesis in Zebrafish Using Zinc Finger Nucleases (ZFNs)
- Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae
- Enrichment of PBMCs with monocytes (The Science Advisory Board)
- In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors
- Stable Integration of Large PAC Constructs in Keratinocytes
- Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development
- 干细胞的冻存方法与细胞计数
- Purification of Recombinant Poly(ADP-Ribose) Polymerases
- 复合物Ⅱ(complex Ⅱ)