实验方法>
细胞技术>
细胞功能测定>Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay
Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay
关键词: limiting dilution analysis来源: 互联网
Epidermal stem cells are of major importance for tissue homeostasis, wound repair, tumor initiation, and gene therapy. Here we describe an in vivo regeneration assay to test for the ability of keratinocyte progenitors to maintain an epidermis over the long-term in vivo. Limiting dilution analysis of epidermal repopulating units in this in vivo regeneration assay at sequential time points allows the frequency of short-term (transit amplifying cell) and long-term (stem cell) repopulating cells to be quantified.
推荐方法
- Cryopreservation of Mammalian Embryos: Vitrification
- Analysis of Lipid Profiles in Skeletal Muscles
- Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells
- Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Us
- High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis
- Circulating Endothelial Progenitor Cells (CEPC)
- The Coculture Method to Examine Interactions Between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bea
- The Ames Test
- Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe
- Adipose Stem Cell Differentiation into Smooth Muscle Cells