Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells
In our studies of muscle regeneration we have developed, modified, and optimized techniques to isolate and study the stem and precursor cells to muscle tissue. Our goals have been to obtain for study muscle fibers in bulk, or the fiber-associated cells, separately from the other cells found in muscle. Using these techniques, myofiber-associated cells may be isolated from neonatal through adult muscle, from resting or from regenerating muscle, thus allowing one to investigate the cellular populations participating during the time course of these events. The protocol is applicable to any age and condition of muscle and may be adapted for other tissues.
- Isolation of Plasmid DNA: The Cleared Lysate and CsCl Gradient Technique
- 核型(karyotype)
- Joining RNA Molecules with T4 DNA Ligase
- Quantification of Cell Cycle-Arresting Proteins
- Animal Models of Neurodegenerative Diseases
- Measuring the Inflammasome
- Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins
- Stallion Spermatozoa Viability: Comparison of Flow Cytometry with Other Methods
- Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila
- Liposomes for Specific Depletion of Macrophages from Organs and Tissues