Single-Cell Culture in Microwells
In order to better understand cellular processes and behavior, a controlled way of studying high numbers of single cells and their clone formation is greatly needed. This chapter describes a microwell plate with 672 wells in a standard array/slide format, applied for single-cell culture and analysis. Single cells can be seeded into each well of the plate (1) manually or (2) automatically using a sorting flow cytometer, followed by week-long culture and detection of cell growth, protein expression, etc. The glass/silicon plate is compatible with most standard instrumentation to facilitate easy handling and enable use of the plate for single-cell analysis in most laboratory settings.
- 无菌操作技术(细胞培养)
- Evaluation of Individual-Cell Motility
- Grb2蛋白(growth factor receptor-bound protein 2)
- 大鼠铁蛋白(Ferritin)酶联免疫分析
- Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells
- Designing, Testing, and Validating a Microarray for Stem Cell Characterization
- Isolation of Primary Avian Osteocytes
- Measurement of Apoptosis by DNA Fragmentation
- Activation and Function of Small GTPases Rho, Rac, and Cdc42 During Gastrulation
- Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31)