High Throughput Analysis of Nuclear ReceptorCofactor Interactions
Various assays have been employed to study the nuclear receptor/cofactor interactions. Coimmunopre-cipitation protocols, both yeast and mammalian two-hybrid systems, and electrophoretic mobility shift/ supershift assays are all commonly used. One of the most useful assays for studying direct protein—protein interactions is the glutathione-S -transferase “pulldown” assay. We have developed a high-throughput version of this assay that utilizes a filter microplate to allow parallel processing of many samples, significantly reducing the time and reagents required for the assay and increasing the sensitivity of the assay for weaker protein—protein interactions.
- Resuscitation of Frozen Cell Lines
- 联会复合体的染色与观察实验
- A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase
- Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model
- In Vitro and In Vivo Protein Import Into Plant Mitochondria
- Preparative Electrophoresis: Extraction of Nucleic Acids From Gels
- The Dansyl-Edman Method for Peptide Sequencing
- 细胞外基质(extracellular matrix,ECM)
- Testing for Bacteria and Fungi
- Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells