Transfection and DNA-Mediated Gene Transfer
The advent of recent technologies such as gene expression microarrays and high-throughput sequencing methods has allowed for unveiling the molecular complexity of cancer. However, compared to the genomic discovery stage, the functional characterization of genes that have been found altered (by somatic mutations, rearrangements, or copy number variations) or differentially regulated at the expression level is still lagging behind. In the future, it is anticipated that efforts would be aimed at addressing the impact of such genes on several cancer traits, including tumor formation, dissemination, and response to therapies. These studies would likely have to rely on introducing the gene(s) of interest (in its �wild-type or altered version) in cellular models. We describe here a number of techniques to introduce nucleic acids into eukaryotic cells, ranging from conventional plasmid transfection to lentiviral �transduction and adeno-associated viral (AAV)-mediated DNA transfer.
- The Use of Early Sea Urchin Embryos in Anticancer Drug Testing
- Detailed Analysis of Promoter-Associated RNA
- Detection and Analysis of Lung Cancer Cells from Body Fluids Using a Rare Event Imaging System
- Identification of Pancreatic Cancer-Specific Cell-Surface Markers for Development of Targeting Ligands
- Chromatin Immunoprecipitation (ChIP) for Analysis of Histone Modifications and Chromatin-Associated Proteins
- Immunizing Potential of Cytokine-Transduced Tumor Cells
- Regulatory Aspects for Translating Gene Therapy Research into the Clinic
- Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
- 单克隆抗体制备-液体石蜡的处理
- 如何运输细胞