Capillary Electrophoresis of Sugar Acids
This chapter illustrates the usefulness of capillary electrophoresis (CE) for the analysis of sugar acids, that is, monosaccharides and lower oligosaccharides carrying carboxylate, sulphate or phosphate groups. In order to provide a general description of the main results and challenges in the field, some relevant applications and reviews on CE of such saccharidic compounds are tabulated. Furthermore, some detailed experimental procedures are shown, regarding the CE analysis of sugar acids released upon hydrolysis of acidic polysaccharides and of glycans linked to glycoproteins. In particular, the protocols will deal with the following compounds: (i) unsaturated, underivatized oligosaccharides from lyase-treated alginate; (ii) oligosaccharides derivatized with 4-aminobenzonitrile, arising from chemical hydrolysis of alginate; (iii) sialic acid derivatized with 2-aminoacridone, released from human serum immunoglobulin G.
- Isolation and Culture of Epithelial Stem Cells
- Transcriptional Regulation of Epidermal Barrier Formation
- Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media
- The Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Protein Interaction with the GI
- Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye
- Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstream Kinase Activation
- Cloning of Cytokine 3 Untranslated Regions and Posttranscriptional Assessment Using Cell-Based GFP Assay
- Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
- 染色体分带(chromosome banding)
- Assays of MSCs with Microarrays