Generation of Induced Pluripotent Stem Cells from Human Amnion Cells
Induced pluripotent stem (iPS) cells have been generated through nuclear reprogramming of somatic cells via retrovirus- or lentivirus-mediated transduction of exogenous reprogramming factors OCT3/4, SOX2, KLF4 , and c-MYC . The extraembryonic amnion is considered to be a promising candidate cell source for cellular therapeutics and the generation of iPS cells because it contains a large number of cells that do not require any genetic or epigenetic modifications. In this chapter, we describe how to generate human amniotic membrane (hAM) primary cells derived from placenta and how to establish the iPS cells from these hAM cells using four reprogramming factors. The hAM-derived iPS cells could be useful for personal regenerative medicine for future infants and useful as genetic disease models and for disease-specific drug discovery.
- 相变(phase transition)
- 倒置显微镜的操作程序及日常维护需知
- Telomerase Inhibition and Telomere Targeting in Hematopoietic Cancer Cell Lines with Small Non-Nucleosidic Synthetic Compounds (
- Microinjection into Zebrafish Embryos
- Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology
- 三联管(triplet)
- Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques
- Testing Smell When It Is Really Vital: Behavioral Assays of Social Odors in the Neonatal Mouse
- Primary Culture of Mammalian Taste Epithelium
- Sperm DNA Fragmentation Analysis Using the TUNEL Assay