Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting
The sex of mammalian offspring can be predetermined by flow sorting relatively pure living populations of X- and Y-chromosome-bearing sperm. This method is based on precise staining of the DNA of sperm with the nucleic acid-specific fluorophore, Hoechst 33342, to differentiate between the subpopulations of X- and Y-sperm. The fluorescently stained sperm are then sex-sorted using a specialized high speed sorter, MoFlo� SX XDP, and collected into biologically supportive media prior to reconcentration and cryopreservation in numbers adequate for use with artificial insemination for some species or for in vitro fertilization. Sperm sorting can provide subpopulations of X- or Y-bearing bovine sperm at rates in the 8,000 sperm/s range while maintaining; a purity of 90% such that it has been applied to cattle on a commercial basis. The sex of offspring has been predetermined in a wide variety of mammalian species including cattle, swine, horses, sheep, goats, dogs, cats, deer, elk, dolphins, water buffalo as well as in humans using flow cytometric sorting of X- and Y-sperm.
- Ciona Genetics
- Long-Term Culture of Chicken Blastodermal Cells (CBCs) and Selection of Transfected CBCs Using Antibiotic Resistance
- Flow Cytokinetics
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- Reproductive and Developmental Manipulation of the Marsupial, the Tammar Wallaby Macropus eugenii
- Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics
- In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages
- 银染核仁形成区与近端着丝粒染色体随体联合
- 动物细胞培养及外源基因的导入
- Immunodetection of cyclin D1 and D2/D3 using flow cytometry