Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes
Unlike many of the other topics covered in this book, the difficulty in identifying conserved checkpoint genes lies more in choosing an appropriate method than in carrying out the work. Thus, this chapter concentrates more on the alternative strategies and under which circumstances the choice of a given method is appropriate. There are essentially three types of strategies for isolating conserved genes of any sort: through deoxyribonucleic acid (DNA)-sequence conservation, protein-structure conservation, and through functional conservation. Each of these approaches can be further subdivided into different techniques that could be applied, and the choice of technique is dependent on the system in which one is working and on the gene one is attempting to isolate.
- Detection of Apoptosis in Cell-Free Systems
- 胞质溶胶(cytosol)
- Grafting Hensens Node
- Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and Martigel Gels
- Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins
- The Isolation of Membranes from Bacteria
- 细胞生物基本方法:复苏
- Definitions and Criteria for Stem Cells
- 染色体显微切割及新进展
- Granzyme A、B介导的凋亡信号通路