In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears
Immunocytochemistry, together with biochemical procedures, has ensured rapid and accurate identification of tissue components. This has led to a better appreciation of cellular events, particularly storage and secretion of products. However, such techniques have certain drawbacks, in particular the impossibility of monitoring the intracellular processes concerned with protein synthesis. Thus, there was a need for a method that would provide more detailed information about the functional morphology and gene expression in tissues at cellular level. Advances in molecular biology allowed the development of in situ hybridization, a procedure that localizes specific nucleotide sequences (DNA or RNA) in tissue preparations using labeled complementary probes (DNA or RNA).
- Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization
- 磷脂交换蛋白(phospholipid exchang proteins, PEP)
- Purification and Mass-Spectrometry Identification of Microtubule-Binding Proteins from Xenopus Egg Extracts
- Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique
- Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells
- The Myc World Within Reach
- Microalgal Immobilization Methods
- 血清使用过程中的QA
- 分化抑制(differential inhibition)
- 细胞组分和细胞器――细胞器分离