Immunostaining of Germline Stem Cells and the Niche in Drosophila Ovaries
Stem cells have the ability to switch between proliferative (self-renewal) and differentiation modes. The Drosophila germarium is a well-established in vivo model for the study of communication between stem cells and their niche. One commonly used technique for such study is immunostaining that allows examination of protein localization at a fixed time point. This chapter provides a detailed protocol for immunofluorescence staining of Drosophila ovaries. This protocol has been optimized to enable explicit visualization of the niche structure, as well as to maximize the degree of multiplexing for protein labeling and detection.
- Derivation of Contractile Smooth Muscle Cells from Embryonic Stem Cells
- Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos
- Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs
- In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Pro
- Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions
- Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
- 线粒体的活体染色及电镜照片观察实验
- 骨髓间质干细胞的分离和培养
- Immunodetection of cyclin D1 and D2/D3 using flow cytometry
- 细胞介导细胞毒作用检测法3:LDH释放法