Protocols for Nuclear Transfer in Mice
Cloning by nuclear transfer in mammals has revealed the remarkable ability of an oocyte to reprogram transferred cell nuclei and induce them to recapitulate the developmental program. This chapter summarizes the method used since 1998 for mouse cloning, which differs from that for large animal cloning. A Piezo-drill micromanipulator allows direct injection of nuclei into enucleated mice oocytes instead of the electrofusion method used for introducing a nucleus into an enucleated oocyte of a large animal. After activation, reconstructed embryos are allowed to develop to the morulae or blastocyst stage before transfer into surrogate mothers.
- In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells
- Microarray Technology for Mutation Analysis of Low-Template DNA Samples
- Analyzing Planar Cell Polarity During Zebrafish Gastrulation
- Collection, Processing, and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine
- The Corneal Pocket Assay
- 荧光激活的细胞分类仪检测调亡细胞
- Isolation and Characterization of Primitive Hematopoietic Cells Based on Their Position in the Cell Cycle
- Cell-Based Microarrays: Overview
- Intracellular Delivery of Nanoparticles with CPPs
- 协同运输(cotransport)