Isolation and Culture of Mouse Intestinal Cells
Complex cell signal transduction mechanisms regulate intestinal epithelial shape, polarity, motility, organelles, cell membrane components as well as physical and mechanical properties to influence alimentary digestion, absorption, secretion, detoxification and fluid balance. Interactions between the epithelial cells and adjacent mesenchyme are central to intestinal homeostasis although the key regulatory molecules of specific differentiation steps remain unclear. Isolation and primary culture of heterotypic murine intestinal cells provides a model system for elucidation of essential molecular cross-talk between epithelium and mesenchyme that may provide several biological and practical advantages over transformed cell lines. An in vitro primary culture system for neonatal rat or mouse intestinal cells has been established that forms monolayers, expresses intestine-specific epithelial features including intestinal brush borders and appropriate hydrolase enzymes. Our studies confirm the promise of this method which may advance our understanding of heterotypic cellular interactions implicated in intestinal function and may provide important insights into the pathobiology of disease.
- Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis
- The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components
- Serum-Free Culture of Mid-gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs
- DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules
- Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies
- Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown
- BrdU法细胞周期检测实验原理与操作步骤
- 人类巴氏小体的观察
- 翻转酶(flippase)
- CELL ADHESION