Angiogenesis Assays in the Chick CAM
The growth of new blood vessels from pre-existing vascular elements, or angiogenesis, involves coordinated signals to the adhesion, migration, and survival machinery within the target endothelial cell. Agents that interfere with any of these processes may therefore influence angiogenesis. Here, we describe the angiogenesis assay in the chick chorioallantoic membrane (CAM). The CAM is a useful tool to studying angiogenesis because 1) it is amenable to both intravascular and topical administration of study agents, 2) it is a relatively rapid assay, and 3) it can be adapted very easily to study angiogenesis-dependent processes, such as tumor growth. Importantly, the CAM provides a physiological setting that permits investigation of pro- and anti-angiogenic agent interactions in vivo.
- 相变(phase transition)
- Live-Cell Imaging of the Cytoskeleton and MitochondrialCytoskeletal Interactions in Budding Yeast
- Noncovalent pADPr Interaction with Proteins and Competition with RNA for Binding to Proteins
- Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements During Xenopus Gastrulation
- Immunohistochemical Analysis of mTOR Activity in Tissues
- Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins
- Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
- Liposomal siRNA Delivery
- 中心体
- Subculture of Suspension Cell Lines