In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression
This chapter describes methods that allow researchers to localize sites of gene expression at both the mRNA and protein levels, within a histological section of a single tissue or tissue microarrays. Identification of the cells within a tumor specimen that express a specific mRNA (assessed by hybridization in situ ) or protein (assessed by immunohistochemistry) is a significant step toward understanding tumor behavior that is not possible using methods in which tissues are homogenized before analysis. Combined detection of mRNA and protein may permit effects of subtle regulatory processes such as translational repression to be observed and allow coexpression of genes to be detected. Application of such combined techniques to tissue microarrays of tumor tissues from cohorts of patients with known clinical outcome would allow the predictive value of specific patterns of expression to be tested retrospectively.
- Gene Expression Profiling to Characterize Anticancer Drug Sensitivity
- Functional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination Assay
- Immunodiagnosis of Childhood Malignancies
- Assaying the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae
- Solving Problems in Multiplex FISH
- Use of the Polymerase Chain Reaction Technique to Detect the t(14;18) Translocation in Lymphoid Tissue
- Strategies for the Use of Site-Specific Recombinases in Genome Engineering
- Chromosome Preparations from Bone Marrow in Acute Lymphoblastic Leukemia: Cytogenetic Techniques
- The NF-B Transcription Factor Pathway as a Therapeutic Target in Cancer: Methods for Detection of NF-B Activity
- 淋巴细胞分离的关键点