Preparation and Testing of Synthetic mRNA for Microinjection
The misexpression and overexpression of proteins by injection of synthetic mRNA into Xenopus oocytes and, more particularly, embryos has provided the experimental basis for a considerable portion of our current knowledge of early vertebrate development (1 , and references therein). The original experiments performed were designed to test the function of proteins in early development by expressing large quantities of the protein itself both in the cells and at the times when it would normally be expressed (overexpression) and in cells or at times when it would not (ectopic expression). These experiments was used to test the roles of both transcription factors and signaling molecules in Xenopus embryos.
- Isolation and Characterization of Side Population Cells
- 胞质溶胶(cytosol)
- Fluorescence Activated Cell Sorting (FACS)
- Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs
- Optimizing Stem Cell Grafting into the CNS
- An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1
- Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA)
- An Integrative Procedure for Apoptosis Identification and Measurement
- Simultaneous Fluorescence Immunostaining and FISH
- 活细胞培养观察方法