Karyotyping Lymph Node Biopsies in Non-Hodgkins Lymphoma
Culture and transport methods are described that allow chromosomes to be obtained from lymph node biopsies with a high success rate, even when the biopsy is taken at a distant site from the cytogenetic laboratory. Optimal banding techniques for the identification of these chromosomes are given, along with hints on how to correct any problems encountered on initial staining. We also describe how to prepare this material for fluorescence in situ hybridization techniques, both for further delineation of complex chromosomal rearrangements and for interphase analysis in cases in which conventional cytogenetics has not been successful.
- Instrumentation, Accuracy, and Quality Control Issues in Development of Quantitative Fluorescence-Image Analysis (QFIA)
- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Ti
- Gene Expression Profile Analysis of Tumors
- Tumor Suppressor Genes in Lung Cancer
- Flow Cytometric Analysis of MDM2-Mediated Growth Arrest
- Hormonal and Differentiation Agents in Cancer Growth Suppression
- Cytogenetic Studies Using FISH: Background
- Conversion Technology and Cancer Predispositions
- 乳糖操纵子(Lac Operon)
- Markers for Anti-cytotoxic T-lymphocyte Antigen 4 (CTLA-4) Therapy in Melanoma