Isolation of Inflammatory Cells from Human Tumours
Inflammatory cells are present in many tumours, and understanding their function is of increasing importance, particularly to studies of tumour immunology. The tumour-infiltrating leukocytes encompass a variety of cell types, e.g. T lymphocytes, macrophages, dendritic cells, NK cells, and mast cells. Choice of the isolation method greatly depends on the tumour type and the leukocyte subset of interest, but the protocol usually includes tissue disaggregation and cell enrichment. We recommend density centrifugation for initial enrichment, followed by specific magnetic bead negative or positive panning with leukocyte and tumour cell selective antibodies.
- Differential Staining Cytotoxicity Assay: A Review
- Drug Ratio-Dependent Antagonism: A New Category of Multidrug Resistance and Strategies for Its Circumvention
- Adhesion of Tumor Cells to Matrices and Endothelium
- Pathological Staging of Melanoma
- Detection of Telomerase Enzyme Activity by TRAP Assay
- 9 Intracellular Single-Chain Antibodies for Gene Therapy
- Silicon Nanowire Biosensor for Ultrasensitive and Label-Free Direct Detection of miRNAs
- 细胞内钙离子浓度测定的问题
- 成人干细胞和再生(第四部分)
- 真核细胞表达系统