Quantifying Hematopoietic Stem and Progenitor Cell Mobilization
Allogeneic donor blood cells and autologous peripheral blood leukocytes (PBL), obtained following �clinical mobilization procedures, are routinely used as a major source of hematopoietic stem and progenitor cells (HSPC) for transplantation protocols. It is, therefore, essential to evaluate and to quantify the extent by which the HSPC are mobilized and enriched in the circulation in correlation with their long-term hematopoietic reconstitution capacity. In this chapter, we describe quantitative methods that measure the number of mobilized HSPC according to specific criteria, as well as their functional properties in vitro and in vivo . The described assays are useful for assessment of progenitor cell mobilization as applied to both human and murine HSPC.
- Review of Human DC Subtypes
- The Double Immunodiffusion Technique: Immunoprecipitation and Analysis of Antigenic Protein in Gel
- Mathematics of Experimentally Generated Chemoattractant Gradients
- Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy
- Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study
- Purification and Identification of Protein Complexes That Control the Cell Cycle
- 重链结合蛋白(heavy-chain binding protein, Bip)
- Culture Systems: Embryo Density
- 生物安全柜安全性能解惑
- 果蝇细胞的培养实验方法(Culturing Drosophila cells)