Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes
Immobilization is designed to restrict the freedom of movement of an enzyme and, in doing so, places limitations on the enzyme and the biotransformation catalyzed by the enzyme (see Chapter 1 ). In practical terms, this often means that the normal procedures used for assay of the soluble enzyme activity, protein content, and so on, must be redesigned to accomodate the presence of the support material. In general, problems are likely to be experienced when the active agent in an assay procedure binds directly to the enzyme and measurement is based on the enzyme-reagent complex. Support materials are likely to cause interference in such procedures.
- 常规组织传代培养
- Tumorcytogenetic Diagnostics and Research on Uncultured Blood or Bone Marrow and Smears
- Gene Targeting by Oligonucleotides in Keratinocytes
- Preparation of Human Trophoblast Cells for Culture
- Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions
- Chromatin Immunoprecipitation Assays for Myc and N-Myc
- PARP1 Genomics: Chromatin Immunoprecipitation Approach Using Anti-PARP1 Antibody (ChIP and ChIP-seq)
- 如何选择合适的细胞培养基
- 卵子发生(oogenesis)
- Identification of Homing Peptides Using the In Vivo Phage Display Technology