Gene Expression in Mouse Oocytes by RNA-Seq
关键词: gene expression mouse来源: 互联网
Second-generation sequencers such as the Illumina GAIIx make possible the study of all transcribed loci in a genome across an almost endless dynamic range. Although typical protocols call for starting from at least 1 μg of total RNA, this is not possible when studying small tissues or rare cell types. This chapter explains how to prepare Illumina sequencing libraries from mouse oocytes. The protocol is also suitable for mural and cumulus cells, flow sorted or laser captured cells.
推荐方法
- In Vivo Transplantation of Engineered Human Skin
- Tissue Engineering of Cartilage
- Epidermal Lineage
- Analysis of Ligand-Dependent Nuclear Accumulation of Smads in TGF- Signaling
- A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gels
- Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins
- 外核膜(outer nuclear membrane)
- Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters
- 研究细胞凋亡的几种光镜形态学检测方法比较
- Phospholipase A2 Activity