An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells
This chapter describes the methods we use to derive and characterize human induced pluripotent stem (iPS) cells. We describe in order, first our culture techniques for the starting fibroblast populations and methods for retrovirus preparation and concentration. Subsequently, a detailed iPS derivation protocol suitable for human fibroblast populations is discussed using standard retroviral vectors expressing the classic four or three reprogramming genes. Finally, we elaborate a robust technique for monitoring and identification of potential iPS cells through live staining of reprogrammed cells. We also outline steps for characterization of the resulting iPS cell lines.
- The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells
- Long-Circulating, pH-Sensitive Liposomes
- Metal Analysis With Capillary Zone Electrophoresis
- Solid Tissue Dispersal for Cytokinetic Analyses
- 半桥粒(hemidesmosomes)
- Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells
- 细胞生物学名词解释
- Development of Immortalized Hepatocyte-Like Cells from hMSCs
- 细胞学说
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