Identification of Target Genes of a Yeast Transcriptional Repressor
Xbp1 is a transcriptional repressor with homology to the DNA binding domains of two cell-cycle regulatory transcription factors, Swi4 and Mbp1. Target genes bound and regulated by Xbp1, have been identified using differential display (DD). Because little was known about the regulation or function of Xbp1, we identified genes that were underrepresented in RNAs purified from cells in which Xbp1 was ectopically expressed. Four target genes, including three cyclin genes, were identified by DD and then confirmed by binding studies and Northern analysis. The binding site for Xbp1, previously identified by site selection, was present in these target genes and is conserved phylogenetically.
- Quantitative PCR: A Survey of the Present Technology
- 次缢痕(secondary constriction)
- Isolation and Purification of Vertebrate DNAs
- Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors
- In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase
- Physical Structure of Nuclear Receptor-DNA Complexes
- Differentiation and Characterization of Human MSCs
- Computer-Assisted Interpretation of Triacylglycerols Mass Spectra
- MicroRNA Knock Down by Cholesterol-Conjugated Antisense Oligos in Mouse Organ Culture
- R&D Systems 验证神经祖细胞多能性的试剂盒