请问淋巴细胞分离液分离的淋巴细胞需要设门吗
关键词: 淋巴细胞分离液 淋巴细胞来源: 互联网
【求助】急!请问淋巴细胞分离液分离的淋巴细胞需要设门吗?
参与者:二分之一幸福
分析T细胞亚型,通过淋巴细胞分离液分离的淋巴细胞,需要向白细胞那样设门吗?谢谢!
参与者:liu2050
淋巴细胞分离液分离出来的是单个核细胞而不仅仅是单纯的淋巴细胞,还包括nk等一些其他种类单个核细胞。你要分离T细胞亚型,肯定需要2色以上标记,一种是总t细胞的标记,另外一种才是你需要检测的细胞亚型的标记。用总T的标记来圈门,门里面再显色的细胞才是你需要的细胞,这样的标记方法才能保证你要的T细胞亚型检测的更准确些!
参与者:sherrie660117
用流式检测分离后的淋巴细胞亚群的确需要设门,策群力但不局限于用总T细胞标记设门,如果想看总T细胞占淋巴细胞的比例,就不能这样设门.可以用传统的FSC/SSC设门,也可以用CD45/SSC设门,后者更准确。
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